O-65 - SARS-CoV-2 has five BsaI-BsmBI sites yielding six fragments with unique overhangs

Double digestion of the SARS-CoV-2 genome with the Type IIS enzymes BsaI/BsmBI cuts at 5 recognition sites, producing 6 fragments; all 5 resulting 4-nucleotide overhangs are unique and non-palindromic.