O-66 - SARS-CoV-2's longest BsaI-BsmBI fragment is 7,578 bp (25% of genome) and it lacks two conserved BsaI sites
The longest fragment from BsaI/BsmBI digestion of SARS-CoV-2 is 7,578 bp, 25% of the genome; its restriction map is evenly spaced and lacks two highly conserved BsaI sites found in almost all other lineage B sarbecoviruses.
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Why this is evidence
An evenly-spaced restriction map with an anomalously short longest fragment (25% of genome) and two otherwise-conserved BsaI sites removed reads as designed for assembly — favouring H-43 over H-41/H-42.