EpiStack

H-35 - SARS-CoV-2's restriction map was designed to enable RBD swaps and furin-site insertion at S1-S2

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Properties9
type
hypothesis
id
H-35
state
merged
question
Do the positions of SARS-CoV-2's restriction sites reflect purposeful engineering for RBD/FCS manipulation?
statement
The two BsaI sites flanking the S1 region and RBD were purposefully placed to permit efficient substitution of the receptor-binding domain and insertion of a furin cleavage site at the S1/S2 junction, matching a deliberate chimera-construction design.
merged_into
H-43 - Research-related origin of a laboratory-manipulated virus (engineered lab leak)
source
S-48 - Bruttel, Washburne & VanDongen 2022-23 — Endonuclease fingerprint indicates a synthetic origin
locator
Results (Fig 3A/B)
quote
Having two BsaI sites flanking the S1 region and RBD enables efficient substitutions of receptor binding domains or introduction of an FCS at the S1/S2 junction

The two BsaI sites flanking the S1 region and RBD were purposefully placed to permit efficient substitution of the receptor-binding domain and insertion of a furin cleavage site at the S1/S2 junction, matching a deliberate chimera-construction design.

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