O-94 — Controlled inpatient depletion-repletion design (1 wk 500 mg/day choline run-in, 3 wk deficient vs control, 1 wk repletion). Demonstrates that tissue choline stores deplete on a choline-free diet despite endogenous de novo phosphatidylcholine synthesis. See
Methodology
Controlled inpatient depletion-repletion feeding study: healthy male volunteers hospitalized on a semisynthetic diet devoid of choline but with 500 mg/day choline for a 1-week run-in, then randomized to continued choline (control) vs no choline (deficient) for 3 further weeks, with all subjects re-supplemented in week 5. The diet carried adequate but not excess methionine (and folate), so the endogenous methyl-group and de-novo phosphatidylcholine (PEMT) pathways were not artificially starved. Objective biomarkers: plasma/erythrocyte choline and phosphatidylcholine, serum aminotransferases and other liver/renal tests, serum cholesterol.
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