O-65 - SARS-CoV-2 has five BsaI-BsmBI sites yielding six fragments with unique overhangs
Double digestion of the SARS-CoV-2 genome with the Type IIS enzymes BsaI/BsmBI cuts at 5 recognition sites, producing 6 fragments; all 5 resulting 4-nucleotide overhangs are unique and non-palindromic.
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Why this is evidence
Five BsaI/BsmBI sites giving six fragments with unique, non-palindromic overhangs is idealised for reverse-genetics assembly — the synthetic-fingerprint claim, favouring H-43 over the natural members (H-41/H-42).